60 s primary rat hippocampal neuron culture (Transnetyx)
97
Structured Review
Transnetyx
60 s primary rat hippocampal neuron culture
60 S Primary Rat Hippocampal Neuron Culture, supplied by Transnetyx, used in various techniques. Bioz Stars score: 97/100, based on 396 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/60+s+primary+rat+hippocampal+neuron+culture/Tissue/pm41331138-325-66-88
Average 97 stars, based on 396 article reviews
60 S Primary Rat Hippocampal Neuron Culture, supplied by Transnetyx, used in various techniques. Bioz Stars score: 97/100, based on 396 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/60+s+primary+rat+hippocampal+neuron+culture/Tissue/pm41331138-325-66-88
Average 97 stars, based on 396 article reviews
60 s primary rat hippocampal neuron culture - by Bioz Stars,
2026-10
97/100 stars
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Injection:Article Title: CaBLAM: a high-contrast bioluminescent Ca 2+ indicator derived from an engineered Oplophorus gracilirostris luciferase. Article Snippet: For Ca2+ imaging with KCl-mediated depolarization, Mattek dishes were treated similarly to cell lines, with removal of all but 500 μl of medium, equilibration in the stage-top incubation chamber on the microscope and imaging following the same basic protocol as described above for ionomycin and l-histamine experiments. .. Dark images were recorded for ~30 s, followed by injection of Fz diluted in 1,000 μl of medium at a final concentration of 4.6 μM (1:1,000) and continuous imaging for 60 s. Finally, all neurons were rapidly depolarized by injection of 500 μl of medium supplemented with 120 mM KCl to give a final concentration of 30 mM KCl and images were collected for an additional Concentration Assay:Article Title: CaBLAM: a high-contrast bioluminescent Ca 2+ indicator derived from an engineered Oplophorus gracilirostris luciferase. Article Snippet: For Ca2+ imaging with KCl-mediated depolarization, Mattek dishes were treated similarly to cell lines, with removal of all but 500 μl of medium, equilibration in the stage-top incubation chamber on the microscope and imaging following the same basic protocol as described above for ionomycin and l-histamine experiments. .. Dark images were recorded for ~30 s, followed by injection of Fz diluted in 1,000 μl of medium at a final concentration of 4.6 μM (1:1,000) and continuous imaging for 60 s. Finally, all neurons were rapidly depolarized by injection of 500 μl of medium supplemented with 120 mM KCl to give a final concentration of 30 mM KCl and images were collected for an additional Imaging:Article Title: CaBLAM: a high-contrast bioluminescent Ca 2+ indicator derived from an engineered Oplophorus gracilirostris luciferase. Article Snippet: For Ca2+ imaging with KCl-mediated depolarization, Mattek dishes were treated similarly to cell lines, with removal of all but 500 μl of medium, equilibration in the stage-top incubation chamber on the microscope and imaging following the same basic protocol as described above for ionomycin and l-histamine experiments. .. Dark images were recorded for ~30 s, followed by injection of Fz diluted in 1,000 μl of medium at a final concentration of 4.6 μM (1:1,000) and continuous imaging for 60 s. Finally, all neurons were rapidly depolarized by injection of 500 μl of medium supplemented with 120 mM KCl to give a final concentration of 30 mM KCl and images were collected for an additional |