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60 s primary rat hippocampal neuron culture  (Transnetyx)


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    Structured Review

    Transnetyx 60 s primary rat hippocampal neuron culture
    60 S Primary Rat Hippocampal Neuron Culture, supplied by Transnetyx, used in various techniques. Bioz Stars score: 97/100, based on 396 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/60+s+primary+rat+hippocampal+neuron+culture/Tissue/pm41331138-325-66-88
    Average 97 stars, based on 396 article reviews
    60 s primary rat hippocampal neuron culture - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Injection:

    Article Title: CaBLAM: a high-contrast bioluminescent Ca 2+ indicator derived from an engineered Oplophorus gracilirostris luciferase.
    Article Snippet: For Ca2+ imaging with KCl-mediated depolarization, Mattek dishes were treated similarly to cell lines, with removal of all but 500 μl of medium, equilibration in the stage-top incubation chamber on the microscope and imaging following the same basic protocol as described above for ionomycin and l-histamine experiments. .. Dark images were recorded for ~30 s, followed by injection of Fz diluted in 1,000 μl of medium at a final concentration of 4.6 μM (1:1,000) and continuous imaging for 60 s. Finally, all neurons were rapidly depolarized by injection of 500 μl of medium supplemented with 120 mM KCl to give a final concentration of 30 mM KCl and images were collected for an additional 30–60 s. Primary rat hippocampal neuron culture and Ca2+ imaging Primary E18 rat hippocampal neurons were prepared from tissue shipped from BrainBits (Transnetyx) following the vendor’s protocol. .. Neurons were seeded on poly-d-lysine-coated 18 mm glass coverslips (Neuvitro) in 12-well tissue culture plates (1 × 105 neurons per well) and grown in Gibco Neurobasal Media supplemented with 2% B27, 0.1% gentamycin and 1% GlutaMAX (all from Invitrogen).

    Concentration Assay:

    Article Title: CaBLAM: a high-contrast bioluminescent Ca 2+ indicator derived from an engineered Oplophorus gracilirostris luciferase.
    Article Snippet: For Ca2+ imaging with KCl-mediated depolarization, Mattek dishes were treated similarly to cell lines, with removal of all but 500 μl of medium, equilibration in the stage-top incubation chamber on the microscope and imaging following the same basic protocol as described above for ionomycin and l-histamine experiments. .. Dark images were recorded for ~30 s, followed by injection of Fz diluted in 1,000 μl of medium at a final concentration of 4.6 μM (1:1,000) and continuous imaging for 60 s. Finally, all neurons were rapidly depolarized by injection of 500 μl of medium supplemented with 120 mM KCl to give a final concentration of 30 mM KCl and images were collected for an additional 30–60 s. Primary rat hippocampal neuron culture and Ca2+ imaging Primary E18 rat hippocampal neurons were prepared from tissue shipped from BrainBits (Transnetyx) following the vendor’s protocol. .. Neurons were seeded on poly-d-lysine-coated 18 mm glass coverslips (Neuvitro) in 12-well tissue culture plates (1 × 105 neurons per well) and grown in Gibco Neurobasal Media supplemented with 2% B27, 0.1% gentamycin and 1% GlutaMAX (all from Invitrogen).

    Imaging:

    Article Title: CaBLAM: a high-contrast bioluminescent Ca 2+ indicator derived from an engineered Oplophorus gracilirostris luciferase.
    Article Snippet: For Ca2+ imaging with KCl-mediated depolarization, Mattek dishes were treated similarly to cell lines, with removal of all but 500 μl of medium, equilibration in the stage-top incubation chamber on the microscope and imaging following the same basic protocol as described above for ionomycin and l-histamine experiments. .. Dark images were recorded for ~30 s, followed by injection of Fz diluted in 1,000 μl of medium at a final concentration of 4.6 μM (1:1,000) and continuous imaging for 60 s. Finally, all neurons were rapidly depolarized by injection of 500 μl of medium supplemented with 120 mM KCl to give a final concentration of 30 mM KCl and images were collected for an additional 30–60 s. Primary rat hippocampal neuron culture and Ca2+ imaging Primary E18 rat hippocampal neurons were prepared from tissue shipped from BrainBits (Transnetyx) following the vendor’s protocol. .. Neurons were seeded on poly-d-lysine-coated 18 mm glass coverslips (Neuvitro) in 12-well tissue culture plates (1 × 105 neurons per well) and grown in Gibco Neurobasal Media supplemented with 2% B27, 0.1% gentamycin and 1% GlutaMAX (all from Invitrogen).



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    97
    Transnetyx 60 s primary rat hippocampal neuron culture
    60 S Primary Rat Hippocampal Neuron Culture, supplied by Transnetyx, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/60+s+primary+rat+hippocampal+neuron+culture/Tissue/pm41331138-325-66-88
    Average 97 stars, based on 1 article reviews
    60 s primary rat hippocampal neuron culture - by Bioz Stars, 2026-10
    97/100 stars
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